Plasmid_Backbone

Part:BBa_K5179110:Design

Designed by: Caden Sanko   Group: iGEM24_William-and-Mary   (2024-10-01)


BioBrick Compatible P4 Cosmid


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 6104
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 4100
    Illegal NotI site found at 6110
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 6104
    Illegal BamHI site found at 4623
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 6104
    Illegal suffix found at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 6104
    Plasmid lacks a suffix.
    Illegal XbaI site found at 6119
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NgoMIV site found at 1659
    Illegal AgeI site found at 2901
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.


Design Notes

The BioBrick Compatible P4 Cosmid has had all RFC[10] and RFC[1000] illegal cut sites removed and the BioBrick prefix and suffix added.


Source

Includes the Cos site and Sid operon from bacteriophage satellite P4. DNA sourced from P4 cosmid (Addgene plasmid # 196335), which was a gift from Baojun Wang.

References

Fa-Arun J, Huan YW, Darmon E, Wang B. Tail-Engineered Phage P2 Enables Delivery of Antimicrobials into Multiple Gut Pathogens. ACS Synth Biol. 2023 Feb 17;12(2):596-607. doi: 10.1021/acssynbio.2c00615. Epub 2023 Feb 2. PMID: 36731126; PMCID: PMC9942202.