Coding

Part:BBa_K5178017:Design

Designed by: Yuhang Mao   Group: iGEM24_Virginia   (2024-10-02)


p43-t7rnap


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 75
    Illegal NheI site found at 79
    Illegal NheI site found at 88
    Illegal NheI site found at 2781
    Illegal NheI site found at 2785
    Illegal NheI site found at 2789
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2277
    Illegal XhoI site found at 596
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2710
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1199


Design Notes

Used strong constitutive promoter to increase t7 RNA polymerase production


Source

See basic part

References