Coding

Part:BBa_K5178017

Designed by: Yuhang Mao   Group: iGEM24_Virginia   (2024-10-02)


p43-t7rnap

This composite part combines the coding sequence of T7 RNA Polymerase, a terminator, with a strong constitutive promoter (p43) followed by a lac operator. The coding sequence, T7 RNA polymerase, will be transcribed when the lac operator is unbound in the absence of lacI.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 75
    Illegal NheI site found at 79
    Illegal NheI site found at 88
    Illegal NheI site found at 2781
    Illegal NheI site found at 2785
    Illegal NheI site found at 2789
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2277
    Illegal XhoI site found at 596
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2710
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1199


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Categories
Parameters
None