Coding

Part:BBa_K5178015

Designed by: Virginia iGEM 2024   Group: iGEM24_Virginia   (2024-10-02)


T7RNAP_B0015

The part contains a coding sequence, optimized for B. subtilis, for T7 RNA polymerase. The sequence also contains a double terminator (one T7RNAP-specific, and one E. coli terminator proven to work in B. subtilis). T7 RNA polymerase is a high-fidelity, efficient polymerase.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2656
    Illegal NheI site found at 2660
    Illegal NheI site found at 2664
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2152
    Illegal XhoI site found at 471
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2585
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1074


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Categories
Parameters
None