Regulatory

Part:BBa_K5178002

Designed by: Kaylin Shukert, Hannah Shen, Elizabeth MacPhail   Group: iGEM24_Virginia   (2024-10-01)


p43, cre binding sites version B

p43 promoter, two identical cre binding sites (identified from amyE gene in B. subtilis). This part suppresses transcription of downstream genes when the Ccpa-HPrSerP complex binds to the cre sites. The CcpA-HPrSerP complex forms in the presence of extracellular glucose.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/prokaryote/subtilis
//regulation/negative
Parameters
chassisBacillus subtilis
negative_regulatorsGlucose, and other carbohydrates