Part:BBa_K5175036:Design
T7 promoter - tphB-tpaK-T7 terminator
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 1906
Illegal PstI site found at 2195 - 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 2462
Illegal PstI site found at 1906
Illegal PstI site found at 2195 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 242
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 1906
Illegal PstI site found at 2195 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 1906
Illegal PstI site found at 2195
Illegal NgoMIV site found at 289
Illegal NgoMIV site found at 964 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
It is a composite component consisting of the T7 promoter, T7 terminator, target genes tphB, tpaK. It is responsible for oxidising the diol moiety (two hydroxyl groups) of DCD to a keto group to result in the production of PCA and transporting TPA. TphB is a dehydrogenase that oxidises the diol moiety (two hydroxyl groups) of DCD to a keto group, resulting in the production of PCA. TpaK is the TPA transporter protein.
Source
tphB is from Comamonas sp.E6. tpaK is from Rhodococcus erythropolis.
References
[1] SASOH M, MASAI E, ISHIBASHI S, et al. Characterization of the Terephthalate Degradation Genes of Comamonas sp. Strain E6 [J]. Applied and Environmental Microbiology, 2006, 72(3): 1825-32.
[2] KINCANNON W M, ZAHN M, CLARE R, et al. Biochemical and structural characterization of an aromatic ring-hydroxylating dioxygenase for terephthalic acid catabolism [J]. Proc Natl Acad Sci U S A, 2022, 119(13): e2121426119.
[3] HOSAKA M, KAMIMURA N, TORIBAMI S, et al. Novel tripartite aromatic acid transporter essential for terephthalate uptake in Comamonas sp. strain E6 [J]. Appl Environ Microbiol, 2013, 79(19): 6148-55.