Coding

Part:BBa_K5175008:Design

Designed by: Xihong Zeng   Group: iGEM24_HUST-China   (2024-09-29)


tphA1


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 79
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 954
    Illegal PstI site found at 79
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 79
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 79
    Illegal NgoMIV site found at 394
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

TphA1 contains a [2Fe -2S] iron-sulfur cluster and a flavin adenine dinucleotide (FAD) binding site that transfers electrons from an electron donor (e.g., NADPH) to the oxidized component of TPADO.
TphA1 does not directly participate in the oxygenation reaction.


Source

Comamonas sp. E6

References

[1] KINCANNON W M, ZAHN M, CLARE R, et al. Biochemical and structural characterization of an aromatic ring-hydroxylating dioxygenase for terephthalic acid catabolism [J]. Proc Natl Acad Sci U S A, 2022, 119(13): e2121426119.