Coding

Part:BBa_K5175004:Design

Designed by: Xihong Zeng   Group: iGEM24_HUST-China   (2024-09-29)


aldA


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 695
    Illegal PstI site found at 1408
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 695
    Illegal PstI site found at 1408
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 233
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 695
    Illegal PstI site found at 1408
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 695
    Illegal PstI site found at 1408
    Illegal AgeI site found at 505
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

aldA is the gene for aldehyde dehydrogenase A, which is an enzyme with a relatively broad substrate specificity for small hydroxyaldehyde substrates and can convert glycolaldehyde (GLA) to glycolic acid (GA).
Aldehyde dehydrogenase A is an enzyme with a relatively broad substrate specificity for small hydroxy aldehyde substrates.

Source

E.coli

References

[1]ZHU Y, LIN E C. Loss of aldehyde dehydrogenase in an Escherichia coli mutant selected for growth on the rare sugar L-galactose [J]. J Bacteriol, 1987, 169(2): 785-9.