Coding

Part:BBa_K5175003:Design

Designed by: Zhengdong Wu   Group: iGEM24_HUST-China   (2024-09-29)


fucO


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 167
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 167
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 167
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 167
    Illegal AgeI site found at 883
    Illegal AgeI site found at 1084
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

fucO is the gene for L-1,2-propanediol oxidoreductase, which is an iron-dependent group III dehydrogenase and can convert Ethylene glycol(EG) to glycolaldehyde (GLA).
L-1,2-propanediol oxidoreductase is an iron-dependent group III dehydrogenase.
EG is first converted in E.coli to glycolaldehyde (GLA) by L-1,2 -propylene glycol oxidoreductase.

Source

E.coli

References

[1]MONTELLA C, BELLSOLELL L, PéREZ-LUQUE R, et al. Crystal structure of an iron-dependent group III dehydrogenase that interconverts L-lactaldehyde and L-1,2-propanediol in Escherichia coli [J]. J Bacteriol, 2005, 187(14): 4957-66.