RNA

Part:BBa_K5173008:Design

Designed by: Gaocheng Zeng   Group: iGEM24_EHSBNU   (2024-10-02)


optimized α in sf9


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2000
    Illegal XhoI site found at 558
    Illegal XhoI site found at 1690
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We downloaded and optimizied the mature peptide sequence of the alpha subunit because the Sf9 cells have differences in expression mechanisms.


Source

The mature peptide sequence of the alpha subunit was downloaded from NCBI GenBank.

References