Coding

Part:BBa_K5173007:Design

Designed by: Jiayi Chen   Group: iGEM24_EHSBNU   (2024-10-02)


Clotting factor G beta subunit optimized for e.coli expression host


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 485
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We downloaded and optimizied the mature peptide sequence of the beta subunit only because the prokaryotic expression host lacks protein modification mechanisms.


Source

The mature peptide sequence of the beta subunit was downloaded from NCBI GenBank.

References