Plasmid

Part:BBa_K5173001

Designed by: Jiayi Chen   Group: iGEM24_EHSBNU   (2024-09-30)


pET-28a plasmid with optimized tachypleus tridentatus clotting factor G beta subunit

This recombinant pET-28a plasmid has the optimized sequence of tachypleus tridentatus clotting factor G beta subunit inserted into its multiple cloning site. After they are transformed into e.coli BL21 cells by heat shock, the cells would express the recombinant protein subunit under appropriate conditions, so that the recombinant clotting factor G protein may later be produced during purification through combining both its alpha and beta subunits.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 630
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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