Coding

Part:BBa_K5172002:Design

Designed by: Alwin Thomas   Group: iGEM24_Sheffield   (2024-09-29)


The redesigned version of PafA, 50.2 (F. johnsoniae)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Additional Considerations The variant was designed with specific conserved residues fixed to enhance stability and activity. During the redesign process, emphasis was placed on residues near the active site, utilising ConSurf analysis for evolutionary conservation.



Source

Redesigned PafA from flavobacterium johnsoniae (BBa_K5172000) using LigandMPNN (see our wiki for details).


References