Part:BBa_K5171000:Design

IsPETase-HiCutinase Fusion Enzyme

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI site found at 426
  

Design Notes

Due to the high complexities in the original linker sequence, we modified certain base pair to ensure that it could be successfully manufactured.

Source

This is a composite part including the (GGGGS)3 linker sequence encoded in part BBa_K3819072, the PETase coding sequence encoded in part BBa_K2010000 and the cutinase coding sequence encoded in part BBa_K1602018.