Part:BBa_K5170006
Phosphomimetic Tau compatible with pET29b vector
Study of several phosphopathies require specific phosphorylation of certain proteins, which is extremely hard to accomplish practically using random phosphorylation protocols. Study of Tauopathies had been highly restricted because of this shortcoming of the field. However, a much recent development has shown the use of phosphomimetics; replacement of phosphorylable aminoacids (Threonine, Tyrosine and Serine) by Glutamic acid or Aspartic acid, which resemble the charge distribution or structure of a phosphorylation.
The hyperphosphorylation of Tau causes misfolding, oligomerisation and aggreagation, being an unisolable factor in gradual loss of neuron. A few sites have credible statistical and clinical relevance in case of Tauopathies including Alzheimer's disease: 181T, 198S, 199S, 217T, 231T, 396S, 404S, 422S etc. Thus, we changed these particular sites correspondingly in hTau441 sequence which is compatible with pET29b vector plasmid vector.
Usage and Biology
Sites 181T, 198S, 217T, 231T, 396S, 404S and 422S are mutated to phosphomimetic equivalent. The key followed is Serine replaced with Aspartic acid and Threonine replaced with Glutamic acid. This can be used explicitly for studying Alzheimer's like condition in vitro, predicting thermodynamic properties of pTau (process of aggregation) etc, which can help to understand Tauopathy better in the coming future.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 595
Illegal PstI site found at 1180 - 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 41
Illegal PstI site found at 595
Illegal PstI site found at 1180 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 113
Illegal BamHI site found at 137 - 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 595
Illegal PstI site found at 1180 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 595
Illegal PstI site found at 1180 - 1000COMPATIBLE WITH RFC[1000]
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