DNA
IT3

Part:BBa_K5170002

Designed by: Teng, I-Ting., Li, X., Yadikar, H. A., Yang, Z., Li, L., Lyu, Y., Pan, X., Wang, K. K., & Tan, W.   Group: iGEM24_IISc-Bengaluru   (2024-10-01)


DNA Aptamer against 231pTau

Several statistics from analysis of AD and normal brain suggest that there exist a few phosphorylation site biomarkers in case of Tauopathies, however they cannot be solely trusted for diagnosis of the disease from quantities present in blood. Therapeutically, aptamers show the potential of structural specificity better than antibodies although the binding affinity maybe several orders less.

231 Threonine site in hTau 441 has been closely associated with Alzheimer's Disease. Thus, this aptamer selected for 231pTau can be simply used to study direct application in AD models, conjugated with fluorophores, developed into PROTACs or LYTACs etc as a use in projects.


Usage and Biology

5'CAGCACCGTCAACTGAAT3' and 5'GTGATGCGATGGAGATGT3' are constant regions in the two ends of the sequence. The binding region is in the middle, which has high frequency of G.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 43
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None