Composite

Part:BBa_K5166040:Design

Designed by: Sihui Liu; Yancheng Zeng; Xiao Han; Zhirong Wang   Group: iGEM24_BIT-China   (2024-10-01)


pGAPZα-mntR-pir


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1677
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1174
    Illegal NotI site found at 1663
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 730
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1677
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1677
    Illegal AgeI site found at 1783
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 238


Design Notes

No


Source

We designed the part.

References

[1] SK Amir Hossain;SM Rifat Rahman;Toufiq Ahmed;Chanchal Mandal.An overview of yeast cell wall proteins and their contribution in yeast display system[J].Asian Journal of Medical and Biological Research,2019,Vol.5(4): 246-257
（https://www.researchgate.net/publication/339288607_An_overview_of_yeast_cell_wall_proteins_and_their_contribution_in_yeast_display_system）
[2] Andreu, C.1;del Olmo, M.2.Yeast arming systems: pros and cons of different protein anchors and other elements required for display(Review)[J].Applied Microbiology and Biotechnology,2018,Vol.102(6): 2543-2561
（https://link.springer.com/article/10.1007/s00253-018-8827-6）
[3] Tabanag, IDF (Tabanag, Ian Dominic Flormata)1;Chu, IM (Chu, I-Ming)2;Wei, YH (Wei, Yu-Hong)3;Tsai, SL (Tsai, Shen-Long)1.The Role of Yeast-Surface-Display Techniques in Creating Biocatalysts for Consolidated BioProcessing[J].Catalysts,2018,Vol.8(3): 94
（https://www.mdpi.com/2073-4344/8/3/94）
[4] Kouichi Kuroda;Ken Matsui;Shinsuke Higuchi;Atsushi Kotaka;Hiroshi Sahara;Yoji Hata;Mitsuyoshi Ueda.Enhancement of display efficiency in yeast display system by vector engineering and gene disruption.[J].Applied Microbiology & Biotechnology,2009,Vol.82(4): 713-719
（https://pubmed.ncbi.nlm.nih.gov/19123001/）
[5] Tanaka, Tsutomu 1;Matsumoto, Sayoko 1;Yamada, Mari 1;Yamada, Ryosuke 2;Matsuda, Fumio 2;Kondo, Akihiko 1.Display of active beta-glucosidase on the surface of Schizosaccharomyces pombe cells using novel anchor proteins.[J].Applied Microbiology & Biotechnology,2013,Vol.97(10): 4343-4352
（https://link.springer.com/article/10.1007/s00253-013-4733-0）