Generator

Part:BBa_K516221:Experience

Designed by: Susanna Zucca, Federica Sampietro, Viola Ghio   Group: iGEM11_UNIPV-Pavia   (2011-09-08)

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K516221

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UNIPV-Pavia iGEM 2011

NB: unless differently specified, all tests were performed in E. coli MGZ1 in M9 supplemented medium at 37°C in low copy plasmid pSB4C5.

Characterized with:

The AiiA enzyme activity has been characterized under the regulation of ptet promoter, assaying its enzymatic activity.
The four measurement parts pTet-RBSx-AiiA-TT were assayed by BBa_T9002 biosensor - see experience page.
No HSL degradation was observed for the tested devices in low copy plasmid pSB4C5 after 21 h in M9 medium with pH=7.0.
A significant HSL degradation was observed in E. coli TOP10 in high copy number plasmid pSB1A2.
In this case, a significant difference in degradation between pTet-RBSx-AiiA-TT and the negative control was observed, also just after 7 hours. That was the proof of the good functioning (in HIGH COPY) of the enzyme.

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