Generator

Part:BBa_K516210:Design

Designed by: Nicolo' Politi, Susanna Zucca, Daniele Sartori   Group: iGEM11_UNIPV-Pavia   (2011-09-08)

LuxI protein generator with Ptet and RBS B0030 (TERM-)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 720
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

  • The Biobrick Part was designed without a terminator element downstream the luxI coding sequence.
    Nevertheless, it is possible use this part into plasmid vectors (such as pSB4C5) containing a Terminator in their backbones.
  • luxI coding sequence is LVA tagged to decrease the protein half-life. (Andersen JB et al. 1998)

Source

Registry

References