Plasmid

Part:BBa_K5160007

Designed by: Guangbin An   Group: iGEM24_SZU-China   (2024-09-25)


TRV1

Overview

Used with TRV RNA2 for virus-induced transient expression to aid in virus transmission in plants.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 446
    Illegal XbaI site found at 631
    Illegal SpeI site found at 5573
    Illegal PstI site found at 4610
    Illegal PstI site found at 5596
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 446
    Illegal SpeI site found at 5573
    Illegal PstI site found at 4610
    Illegal PstI site found at 5596
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 446
    Illegal BglII site found at 1029
    Illegal BglII site found at 5070
    Illegal XhoI site found at 1488
    Illegal XhoI site found at 5689
    Illegal XhoI site found at 6685
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 446
    Illegal XbaI site found at 631
    Illegal SpeI site found at 5573
    Illegal PstI site found at 4610
    Illegal PstI site found at 5596
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 446
    Illegal XbaI site found at 631
    Illegal SpeI site found at 5573
    Illegal PstI site found at 4610
    Illegal PstI site found at 5596
    Illegal AgeI site found at 4009
    Illegal AgeI site found at 5151
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1212
    Illegal BsaI.rc site found at 1540
    Illegal BsaI.rc site found at 1988
    Illegal BsaI.rc site found at 3752
    Illegal BsaI.rc site found at 5429
    Illegal SapI site found at 863

Biology

TRV1 refers to a component of Tobacco rattle virus (TRV), a double-stranded RNA virus whose genome consists of RNA1 and RNA2. The TRV genome is reverse transcribed into cDNA in vitro by the action of a reverse transcriptase enzyme, and the cDNA is specifically modified and cloned into a vector that is non-virulent but has the ability to self-replicate and express exogenous genes.
TRV1 vectors usually contain genes encoding RNA-dependent RNA polymerase (RdRp), movement protein (MP), and 16 kD protein, and are a helper viral vector, whose function is mainly to assist the replication and transmission of the virus in plants.
RNA polymerase: Responsible for the replication of viral RNA and synthesizing new viral RNA molecules.
Movement proteins: Can help the virus to move between plant cells, enabling the virus to infect more cells and extend the range of infection.
16 kD proteins: Functions are not yet fully defined and may play a role in virus-host interactions.
In our project, we injected TRV1 together with TRV2 with the target gene into tomato leaves, and TRV1 and TRV2 would replicate and assemble inside the plant cells to form complete virus particles. As the virus replicates and spreads, the target gene fragment inserted into TRV2 will also be copied in large quantities and accumulate in the plant.



Design

In carrying out the design, we directly injected the vector of TRV1 as a plasmid into plant leaves.



Source

TRV1 is derived from Tobacco rattle virus (TRV). It has been artificially adapted to become a helper virus vector that assists in the replication and spread of the virus in the plant.





[edit]
Categories
Parameters
None