Composite

Part:BBa_K5159028:Design

Designed by: Tianyue WU   Group: iGEM24_HKUST   (2024-10-01)


PEX10 Knockout Cassette


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1035
    Illegal PstI site found at 196
    Illegal PstI site found at 415
    Illegal PstI site found at 4250
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1035
    Illegal PstI site found at 196
    Illegal PstI site found at 415
    Illegal PstI site found at 4250
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1035
    Illegal BglII site found at 3267
    Illegal BamHI site found at 251
    Illegal BamHI site found at 4136
    Illegal XhoI site found at 1981
    Illegal XhoI site found at 2010
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1035
    Illegal PstI site found at 196
    Illegal PstI site found at 415
    Illegal PstI site found at 4250
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1035
    Illegal PstI site found at 196
    Illegal PstI site found at 415
    Illegal PstI site found at 4250
    Illegal NgoMIV site found at 1123
    Illegal AgeI site found at 2595
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1641
    Illegal BsaI site found at 1924
    Illegal BsaI.rc site found at 1839
    Illegal SapI site found at 2392


Design Notes

We choose to include two 1000 bp homologous arms in length that are necessary for the efficient knockout to occur in Yarrowia lipolytica.


Source

Please refer to the sources of each subparts.

References