DNA

Part:BBa_K5159015:Design

Designed by: Tianyue WU   Group: iGEM24_HKUST   (2024-09-29)


Y. lipolytica KU70 3' homology arm


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 304
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 304
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 304
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 304
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

A 1000 bp homologous arm in length is necessary for the efficient knockout to occur.


Source

This part sequence is identified in the Yarrowia lipolytica genome and in our project synthesized by commercial provider GenScript.

References