Part:BBa_K5159007
Yarrowia lipolytica LEU2 gene
The LEU2 gene encodes Beta-isopropylmalate dehydrogenase (IMDH) in Yarrowia lipolytica, which catalyzes the third step in the leucine biosynthesis pathway, and can additionally catalyze the conversion of beta-ethylmalate into alpha-ketovalerate.
The expression of the LEU2 gene serves as an auxotrophic selection marker for Yarrowia lipolytica grown on plates without essential amino acid Leucine (Leu-), as only cells transformed to express this gene can synthesis leucine and survive under the leucine-auxotrophic condition, while other untransformed cells are unable to grow.
The gene is expressed under the native LEU2 promoter and terminator, forming the LEU2 expression cassette (Part:BBa_K5159020). This cassette can be flanked with 2 LoxP sites, enabling it to be released by the Cre recombinase system and used for the next rounds of transformation selection.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 656
Illegal XhoI site found at 685 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 316
Illegal BsaI site found at 599
Illegal BsaI.rc site found at 514
Illegal SapI site found at 1067
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