Regulatory

Part:BBa_K5159005:Design

Designed by: Tianyue WU   Group: iGEM24_HKUST   (2024-09-26)


Yarrowia lipolytica TEF promoter with intron


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2


Design Notes

An intron sequence is included to increase the promoter strength. To make the part RCF[10] compatible, all EcoRI, XbaI, SpeI and PstI cut sites were also avoided in the sequence.


Source

This part sequence is identified in the Yarrowia lipolytica genome and in our project synthesized by commercial provider Integrated DNA Technology (IDT)

References