Part:BBa_K5154016

lexA-Rlm1

lexA fused with ⅔ of Rlm1.

lexA is a bacterial protein involved in SOS response in E.coli, and i could interact with lexA operator/operon-like structure and activate the expression of downstream sequence.

Rlm1 is a transcription factor involved in S. cerevisiae CWI (Cell Wall Integrity) pathway. It will be phosphorylated when there is an osmotic pressure change / mechanical stress around the cell (Levin et al, 2011).

lexA fused with Rlm1 (Watanabe et al, 1997) could act as a synthetic transcription factor and interact with Galp-lexAops to monitor the activation of CWI in S. cerevisiae.

For more testing details with Galp-lexAops, see https://parts.igem.org/Part:BBa_K5154000.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 931
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 931
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 931
  Illegal BglII site found at 537
  Illegal BamHI site found at 607
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 931
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 931
  Illegal AgeI site found at 914
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI site found at 1021
  

References

1. Levin, David E. "Regulation of cell wall biogenesis in Saccharomyces cerevisiae: the cell wall integrity signalling pathway." Genetics 189.4 (2011): 1145-1175.
2. Watanabe, Yasuyuki, et al. "Characterization of a serum response factor-like protein in Saccharomyces cerevisiae, Rlm1, which has transcriptional activity regulated by the Mpk1 (Slt2) mitogen-activated protein kinase pathway." Molecular and cellular biology 17.5 (1997): 2615-2623.