Part:BBa_K5154015

elongated wsc1

elongated wsc1-mid2-histag that extends out of cell wall in S. cerevisiae (Dupres et al, 2010). Wsc1 is a mechanosensor involved in S. ceresiae (Levin et al, 2011), and will activate CWI (Cell Wall Integrity) pathway when there's an osmotic pressure change around the cell.

Proposed structure of the elongated wsc1 located on cell surface membrane (Dupres et al, 2009)

For more testing details, please see

Galp-lexAops testing: https://parts.igem.org/Part:BBa_K5154000

Our engineering cycle: https://2024.igem.wiki/cambridge/engineering

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 1050
  Illegal EcoRI site found at 1266
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 1050
  Illegal EcoRI site found at 1266
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 1050
  Illegal EcoRI site found at 1266
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 1050
  Illegal EcoRI site found at 1266
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 1050
  Illegal EcoRI site found at 1266
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 3
  Illegal SapI site found at 287
  

References

1. Dupres, Vincent, Yves F. Dufrêne, and Jürgen J. Heinisch. "Measuring cell wall thickness in living yeast cells using single molecular rulers." ACS nano 4.9 (2010): 5498-5504.
2. Dupres, Vincent, et al. "The yeast Wsc1 cell surface sensor behaves like a nanospring in vivo." Nature chemical biology 5.11 (2009): 857-862.
3. Levin, David E. "Regulation of cell wall biogenesis in Saccharomyces cerevisiae: the cell wall integrity signalling pathway." Genetics 189.4 (2011): 1145-1175.