Composite

Part:BBa_K5154009

Designed by: Zijiao Wang   Group: iGEM24_Cambridge   (2024-09-29)


F5EPG-pTi_J23109_BN1BCD2_TEVNF5_EPG_CF5_B0015

composite part for the expression of EPG-TEV Fusion with Flexible 5aa linker. Kanamycin Resistant. pTi is used as the backbone.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 822
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal PstI site found at 822
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 872
    Illegal BamHI site found at 1347
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 822
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 822
  • 1000
    COMPATIBLE WITH RFC[1000]


Figure 1 | Plasmid map for BBa_K5154009 on a pTi backbone.


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Categories
Parameters
None