Plasmid

Part:BBa_K5154008

Designed by: Zijiao Wang   Group: iGEM24_Cambridge   (2024-09-29)


TEV_Rep-pSB1C5C_J23119_BCD2HisFuGFP_TEV_REACh2_B0015

Expression plasmid for fuGFP-REACh2 protein. Act as reporter for TEV activity. Chloramphenicol resistant.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1656
    Illegal XhoI site found at 2728
    Illegal XhoI site found at 3620
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 846
    Illegal AgeI site found at 315
  • 1000
    COMPATIBLE WITH RFC[1000]


Figure 1 | Plasmid map for Tev-Rep-psb1c5c-j23119-bcd2hisfugfp-tev-reach2-b0015.


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Parameters
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