Composite

Part:BBa_K5154007

Designed by: Zijiao Wang   Group: iGEM24_Cambridge   (2024-09-29)


T7TE_pTi_T7LacO_BN1BCD2_NlucEPG_T7Term

Composite part for the expression of EPG-Nanoluc (on a pTi backbone). Carries a high strength promoter and Kanamycin resistance.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 96
    Illegal PstI site found at 725
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 725
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 30
    Illegal BglII site found at 775
    Illegal BamHI site found at 1204
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 96
    Illegal PstI site found at 725
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 96
    Illegal PstI site found at 725
    Illegal NgoMIV site found at 285
  • 1000
    COMPATIBLE WITH RFC[1000]

Figure 1 | Plasmid map for BBa_K5154007 on a pTi backbone.


[edit]
Categories
Parameters
None