Composite
Part:BBa_K515107:Design
Designed by: Atipat Patharagulpong Group: iGEM11_Imperial_College_London (2011-09-12)
Composite p(tetR) - Dendra2
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The original Dendra2 sequence had been joint codon optimized for Escherichia coli and Bacillus subtilis and designed to contain the insulator sequence under the influence of a strong RBS. However it could not be synthesised. We therefore allowed Eurofins to codon optimize it for E. coli and only had the coding region synthesised with a prefix and a suffix. Once the gene arrived, we assembled it using standard BioBrick assembly with the TetR repressible promoter (BBa_R0040) and a standard RBS (BBa_R0034).
Source
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