Coding

Part:BBa_K5151018

Designed by: KUO, YUN-HSIN   Group: iGEM24_NYCU-Formosa   (2024-09-01)

GS Linker Introduction

Early detection of diseases is crucial. To achieve this, we can target disease biomarkers for testing to assist in early diagnosis. Currently, clinical tests for disease biomarkers, such as ELISA, RIA, and mass spectrometry, though widely used, tend to require longer detection times. Many have developed biosensors and rapid diagnostic tools (RDT). However, challenges still exist in detecting disease biomarkers, mainly due to the complexity of samples and the insufficient sensitivity and specificity of detection technologies.
To address these issues, our team has proposed a new strategy this year, utilizing a pre-trained Natural Language Processing (NLP) model to identify potential biomarkers for diseases[1]. Through this approach, we aim to overcome the current bottlenecks in detection technologies and provide a more efficient and cost-effective pathway for early diagnosis and timely treatment of diseases. This not only helps to shorten diagnostic times but also improves diagnostic accuracy and patient treatment outcomes.
Lpp-OmpA can express specific proteins on the outer membrane of cells, while the GS Linker can enhance protein folding and stability[2]. Therefore, we plan to use Lpp-OmpA-GS Linker to express receptor proteins on the outer membrane, which will bind to target proteins identified by the model. Subsequently, we will use an electrochemical sensor to detect the expression levels of the target protein, allowing us to determine whether the patient has the disease[3].


More application of this part

Our team has developed a series of Lpp-OmpA platforms and used GS Linkers to connect target proteins for the detection of leukemia-associated diseases.

  1. Lpp-OmpA-GS Linker(BBa_K5151007)
  2. Lpp-OmpA-GS Linker-Protein G(BBa_K5151012)
  3. Lpp-OmpA-GS Linker-GFP-6x His(BBa_K5151009)
  4. Lpp-OmpA-GS Linker-CD55(BBa_K5151010)
  5. Lpp-OmpA-GS Linker-2BOU-2(BBa_K5151006)
  6. Lpp-OmpA-GS Linker-2BOU-3(BBa_K5151015)


Reference

[1] Available at: https://2024.igem.wiki/nycu-formosa/model
[2] Chen, X., Zaro, J., & Shen, W. (2014). Fusion Protein Linkers: Property, Design and Functionality. Adv Drug Deliv Rev, 65(10), 1357–1369. Available at: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3726540/
[3] Available at: https://2024.igem.wiki/nycu-formosa/index.html


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None