Composite

Part:BBa_K5150007

Designed by: Lorena Gallegos Solís, Ana Laura Torres Huerta, Alma Paola Castorena Zorilla, Gabriela Araceli Rosas   Group: iGEM24_TecCEM   (2024-10-01)

MGEs-HIS for B. subtilis


Multipeptide sequence 1 fused with 6xHis tag

The aim of fusing with a 6xHis Tag is simplifying the purification process.AsHistidines have a strong affinity for metal ions like nickel or cobalt, which are immobilized on resin beads in affinity chromatography, this allows the His-tagged protein to selectively bind to the resin while other proteins are washed away.

Figure 1. Diagram of the composite part designed for B.subtilis



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None