Composite

Part:BBa_K5146048:Design

Designed by: Said Laibacher   Group: iGEM24_DUESSELDORF   (2024-10-02)


homology region for K. xylinus BcsA


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 4258
    Illegal PstI site found at 634
    Illegal PstI site found at 4414
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 4258
    Illegal NheI site found at 1461
    Illegal PstI site found at 634
    Illegal PstI site found at 4414
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 4258
    Illegal BglII site found at 3902
    Illegal BamHI site found at 3031
    Illegal XhoI site found at 3910
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 4258
    Illegal PstI site found at 634
    Illegal PstI site found at 4414
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 4258
    Illegal PstI site found at 634
    Illegal PstI site found at 4414
    Illegal NgoMIV site found at 3890
    Illegal AgeI site found at 2703
    Illegal AgeI site found at 3451
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 808
    Illegal SapI site found at 3433


Design Notes

AraE was included to increase chance of success of for inducible system in K. xylinus.


Source

K. xylinus, E. coli, synthetic

References