Plasmid

Part:BBa_K5146045:Design

Designed by: Niklas Küppers, Robin Schüren, Tim Velden   Group: iGEM24_DUESSELDORF   (2024-10-02)


Fixed BBa_J435281 for Golden Gate assemblys after the OYC standard


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 3143
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 741
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1024
    Illegal BsaI.rc site found at 6


Design Notes

We wanted the part to work as intended for Golden Gate Assemblys


Source

BBa_J435281 ORG: Open Yeast Collection, Open Bioeconomy & The FreeGenes Project URL: https://freegenes.github.io/genes/BBF10K_000482.html

References