Plasmid

Part:BBa_K5146045

Designed by: Niklas Küppers, Robin Schüren, Tim Velden   Group: iGEM24_DUESSELDORF   (2024-10-02)


Fixed BBa_J435281 for Golden Gate assemblys after the OYC standard

eforRed selection cassette for level 1 vector, clones into backbone and used for colony selection fixed to work for Golden Gate assembly with Bsa1

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 3143
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 741
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1024
    Illegal BsaI.rc site found at 6


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