Composite

Part:BBa_K5146020:Design

Designed by: Tim Velden   Group: iGEM24_DUESSELDORF   (2024-09-29)


Promoter BBa_J435350 fused to RBS BBa_K2680529 with Promoter MoClo overhangs for Bsa1 assembly


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 100
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 34
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 100
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 100
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The overhangs enable assembly with a 5` UTR


Source

https://parts.igem.org/Part:BBa_J435350 https://parts.igem.org/Part:BBa_K2680529

References