Coding
Mutated ba

Part:BBa_K5145005

Designed by:   Group: iGEM24_CCA-San-Diego   (2024-08-12)


darobactin-resistant bamA

BamA encodes for a vital outer-membrane protein. Sequenced genomes of darobactin-resistant E. coli strains showed mutations in the bamA gene. Researchers at the Lewis Lab of Northeastern University (Imai et. al) sequenced this mutant gene and confirmed the connection between bamA mutations and darobactin resistance.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 340
    Illegal XhoI site found at 2146
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1267
    Illegal AgeI site found at 1786
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None