Coding
Mutated ba
Part:BBa_K5145005
Designed by: Group: iGEM24_CCA-San-Diego (2024-08-12)
darobactin-resistant bamA
BamA encodes for a vital outer-membrane protein. Sequenced genomes of darobactin-resistant E. coli strains showed mutations in the bamA gene. Researchers at the Lewis Lab of Northeastern University (Imai et. al) sequenced this mutant gene and confirmed the connection between bamA mutations and darobactin resistance.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 340
Illegal XhoI site found at 2146 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1267
Illegal AgeI site found at 1786 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |