Coding
darB

Part:BBa_K5145001

Designed by:   Group: iGEM24_CCA-San-Diego   (2024-08-11)


darB

DarB is a gene in the biosynthetic gene cluster that produces darobactin A. It is part of the darABCDE operon. DarBCD encodes for an ABC-type trans-envelope exporter system. DarB and darD encode for the transporter (A membrane fusion protein is encoded by darC). DarB is transcribed with the darA, darC, darD, and darE genes to produce darobactin A. This gene was sequenced and characterized by researchers at the Lewis Lab of Northeastern University (Imai et. al).


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1694
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1694
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1694
    Illegal XhoI site found at 119
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1694
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1694
    Illegal NgoMIV site found at 984
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None