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Part:BBa_K5142048:Design

Designed by: Yuchen Liang   Group: iGEM24_HunanU   (2024-10-02)


Click Virus


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We chose to mutate A27L to reduce the biosafety risk of Click Virus in use. Since the amber suppressor mutations are close to the N-terminus of A27L, premature termination of A27L translation will occur if the artificial translation system is not provided. Therefore, all the progeny of Click Virus after the first round of infection will be A27L-deficiency virus which will be trapped in the target cells due to impaired ability of dissemination.


Source

This part is derived from Orthopoxvirus vaccinia Tian Tan (AF095689.1) by mutating 3 codons in A27L gene to amber suppressor codons (TAG) and translating them to 4-azido-L-phenylalanine using an artificial translation system.

References