Coding

Part:BBa_K5142032:Design

Designed by: Yuchen Liang   Group: iGEM24_HunanU   (2024-09-30)


Vaccinia virus A27L-3stop


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 354
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal SpeI site found at 355
    Illegal PstI site found at 369
    Illegal NotI site found at 7
    Illegal NotI site found at 362
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 355
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal XbaI site found at 16
    Illegal SpeI site found at 355
    Illegal PstI site found at 369
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

According to the three-dimensional structure model of the protein, the N-terminus of A27L is located on the surface of the viral particle. After incorporating 4-azido-L-phenylalanine, the azide group can be link to customized ligands by click reaction of azido and dibenzocyclooctyne (DBCO). Please see BBa_K5142006 for more details.


Source

This part is derived from the vaccinia virus A27L (BBa_K5142006) where the codons of three phenylalanines at the N-terminus are changed to the amber suppressor codon AUG.

References