RNA
Part:BBa_K5142008
Designed by: Yuchen Liang Group: iGEM24_HunanU (2024-09-27)
amber suppressor tRNA
Compared to leucine codons and tryptophan codons, the amber codon is one of the stop codons, and its main function is to send a termination signal during protein synthesis, instructing the ribosome to stop adding amino acids. This is a key mechanism to ensure that protein synthesis ends correctly.
Therefore, we use an amber suppressor tRNA, which reads the UAG stop codon, allowing the insertion of 4-L-azido-phenylalanine at this position. In this way, the UAG codon, which would normally cause the termination of protein synthesis, can now encode a non-natural amino acid. With the introduction of an artificial translation system and the addition of 4-L-azido-phenylalanine, we can synthesize a complete A27L protein with an azide group.
[1] Kensaku Sakamoto 1, Akiko Hayashi, Ayako Sakamoto, Daisuke Kiga, Hiroshi Nakayama, Akiko Soma, Takatsugu Kobayashi, Makoto Kitabatake, Koji Takio, Kazuki Saito, Mikako Shirouzu, Ichiro Hirao, Shigeyuki Yokoyama.Site-specific incorporation of an unnatural amino acid into proteins in mammalian cells[EB/OL].2002[2024-9-28].doi: 10.1093/nar/gkf589..
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1
Illegal EcoRI site found at 1137
Illegal SpeI site found at 15
Illegal PstI site found at 1556 - 12INCOMPATIBLE WITH RFC[12]Illegal prefix found in sequence at 1
Illegal suffix found in sequence at 1543
Illegal EcoRI site found at 1137 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1
Illegal EcoRI site found at 1137 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1
Illegal EcoRI site found at 1137
Illegal SpeI site found at 15
Illegal PstI site found at 1556 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1
Illegal EcoRI site found at 1137
Illegal SpeI site found at 15
Illegal PstI site found at 1556 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
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