Coding

Part:BBa_K5140000:Design

Designed by: saki ikebuchi   Group: iGEM24_TUPLS-Japan   (2024-10-01)


melAmut


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 442
    Illegal PstI site found at 1744
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 442
    Illegal PstI site found at 1744
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 442
    Illegal PstI site found at 1744
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 442
    Illegal PstI site found at 1744
    Illegal NgoMIV site found at 1318
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1170
    Illegal BsaI.rc site found at 475


Design Notes

Nothing


Source

Reference The source of the part will be its sequence which was retrieved from National Center for Biotechnology Information.The mutation was inserted based on this paper, with reference to Christine Nicole S. Santos and Gregory Stephanopoulos Melanin-Based High-Throughput Screen for L-Tyrosine Production in Escherichia coli American Society for Microbiology 2007 74(4) 1190-1197 doi:10.1128/AEM.02448-07 [http://aem.asm.org/cgi/content/abstract/74/4/1190 reference].

References