Composite

Part:BBa_K5131007

Designed by: Yu Han   Group: iGEM24_Squirrel-Shanghai   (2024-09-30)


pGEX-GST-nsp5_native-His 

Information of SARS-CoV-2 nsp5 can be seen in BBa_K5131000. We used this part to express and purify SARS-CoV-2 nsp5 in its native state. Sequence design, expression, purification and characterisation of this part can be found in BBa_K5131001.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 1738
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 770
    Illegal XhoI site found at 1733
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1177
    Illegal SapI.rc site found at 167


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Categories
Parameters
None