Composite

Part:BBa_K5131006

Designed by: Yu Han   Group: iGEM24_Squirrel-Shanghai   (2024-09-30)


pGEX-GST-nsp5-His

Information of SARS-Cov-2 nsp5 can be seen in BBa_K5131000. We try to use this part to express and purify SARS-CoV-2 nsp5. Sequence design, expression and purification of this part can be found in BBa_K5131000.However, the molecular weight of the purified protein differs from the expected molecular weight of nsp5 (33.8 kDa). We determined the reason and investigated how the GST tag might affect nsp5 enzymatic activity based on structure prediction. Details can be found in BBa_K5131000.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 1726
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 770
    Illegal XhoI site found at 1721
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1165
    Illegal SapI.rc site found at 167


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Categories
Parameters
None