Part:BBa_K5127018
J23119-iadCDE
This composite part combines the constitutive promotor J23119 and transcriptional unit iadCDE.
Team: BNDS-China 2024
Design
To optimize the IAA degradation efficiency, we constructed a second plasmid that replaces the inducible T7 promoter with the constitutive promoter J23119 (Figure 1). This modification ensures continuous expression of the IAA degradation pathway, potentially enhancing its overall effectiveness.
Figure 1. Plasmid design of J23119-iadCDE. Created by biorender.com.
Figure 2. The agarose gel electrophoresis results of the PCR products of J23119-iadCDE construction. A, materials to construct J23119-iadCDE. B, Golden gate assembly result of J23119-iadCDE construction. The band at 7947bp in (B) indicated the success in plasmid construction.
We require additional time to further characterize and evaluate the degradation function to ensure optimal performance and reliability in our system.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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