Device
Part:BBa_K5127016:Design
Designed by: Chujing Wu Group: iGEM24_BNDS-China (2024-10-02)
Arabinose inducible Lambda Red system with reporter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1941
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1776
Illegal AgeI site found at 3428 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 1758
Design Notes
The pRed-Aspink expresses the Lambda Red recombinase under the control of an arabinose-inducible promoter. The plasmid also constitutively expresses the chromoprotein, AsPink, which serves as a visual marker to confirm the presence of the plasmid in the host cells (Figure 1).
Figure 1. Plasmid design of pRed-Aspink. Created by biorender.com.
Source
E. Coli
References
Byung Jo Yu, Kui Hyeon Kang, Jun Hyoung Lee, Bong Hyun Sung, Mi Sun Kim, & Sun Chang Kim. (2008). Rapid and efficient construction of markerless deletions in the Escherichia coli genome. Nucleic Acids Research, 36(14), e84–e84. https://doi.org/10.1093/nar/gkn359