Coding
Part:BBa_K5127004:Design
Designed by: Yinuo Li Group: iGEM24_BNDS-China (2024-10-01)
iacR
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part is codon optimized for Goldengate assembly (BsaI, BsmBI, BbsI) and expression in E. coli.
Source
Genome of Pseudomonas putida.
References
Wei, W., Liu, Y., Hou, Y., Cao, S., Chen, Z., Zhang, Y., Cai, X., Yan, Q., Li, Z., Yuan, Y., Wang, G., Zheng, X., & Hao, H. (2023). Psychological stress-induced microbial metabolite indole-3-acetate disrupts intestinal cell lineage commitment. Cell Metabolism. https://doi.org/10.1016/j.cmet.2023.12.026