Coding

Part:BBa_K5124001:Design

Designed by: Louise Brown   Group: iGEM24_Exeter   (2024-08-15)


LwCas13a codon opt


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2114
    Illegal BglII site found at 3266
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

6xHis tag added for protein purification.


Source

From Leptotrichia wadei Cas13, was originally identified in Adbudayyeh et al 2017.

References