Coding

Part:BBa_K5124000:Design

Designed by: Louise Brown   Group: iGEM24_Exeter   (2024-08-15)


LbCas12a codon opt


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1461
    Illegal BglII site found at 2198
    Illegal BglII site found at 3210
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 3112
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The DNA sequence has been mutated to remove forbidden restriction sites to be compatible with BioBrick and Type IIS assembly


Source

Lachnospiraceae bacterium ND2006 (Zetsche et al., 2015)

References