Coding

Part:BBa_K512001:Design

Designed by: Percy Genyk   Group: iGEM11_USC   (2011-09-27)


casABCDE12


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 84
    Illegal PstI site found at 1253
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 84
    Illegal PstI site found at 1253
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3094
    Illegal BglII site found at 4279
    Illegal BamHI site found at 23
    Illegal BamHI site found at 3224
    Illegal BamHI site found at 3585
    Illegal BamHI site found at 4482
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 84
    Illegal PstI site found at 1253
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 84
    Illegal PstI site found at 1253
    Illegal NgoMIV site found at 5203
    Illegal AgeI site found at 784
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1764


Design Notes

Blunt end cloning used.


Source

Cloned from DH5a.

References